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Figure 4: ZIKV stabilizes <t>APP</t> proteins. a. HEK293 cells were transfected with human APP695 expression plasmid, <t>pCAX-APP695,</t> or vector control. Cells were infected with or without ZIKV at 1 MOI overnight. Western blots were used for detection of APP and Tubulin simultaneously. b. Relative levels of APP proteins (APP/Tubulin) in HEK293 cells are shown from three independent
Pcax Flag App, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc pcax flag app plasmids
Figure 4: ZIKV stabilizes <t>APP</t> proteins. a. HEK293 cells were transfected with human APP695 expression plasmid, <t>pCAX-APP695,</t> or vector control. Cells were infected with or without ZIKV at 1 MOI overnight. Western blots were used for detection of APP and Tubulin simultaneously. b. Relative levels of APP proteins (APP/Tubulin) in HEK293 cells are shown from three independent
Pcax Flag App Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcax+flag+app+addgene/pCAX+APP+FLAG+(Plasmid+%2330155)/pmc06562731-59-3-8
Average 86 stars, based on 1 article reviews
pcax flag app plasmids - by Bioz Stars, 2026-09
86/100 stars
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Figure 4: ZIKV stabilizes APP proteins. a. HEK293 cells were transfected with human APP695 expression plasmid, pCAX-APP695, or vector control. Cells were infected with or without ZIKV at 1 MOI overnight. Western blots were used for detection of APP and Tubulin simultaneously. b. Relative levels of APP proteins (APP/Tubulin) in HEK293 cells are shown from three independent

Journal: Journal of Biological Chemistry

Article Title: Amyloid precursor protein is a restriction factor that protects against Zika virus infection in mammalian brains

doi: 10.1074/jbc.ra120.015165

Figure Lengend Snippet: Figure 4: ZIKV stabilizes APP proteins. a. HEK293 cells were transfected with human APP695 expression plasmid, pCAX-APP695, or vector control. Cells were infected with or without ZIKV at 1 MOI overnight. Western blots were used for detection of APP and Tubulin simultaneously. b. Relative levels of APP proteins (APP/Tubulin) in HEK293 cells are shown from three independent

Article Snippet: The pCAX FLAG APP (Addgene plasmid # 30154) is a FLAG-tagged APP expression plasmid APP and pCAX APP 695 (Addgene plasmid # 30137), an expression plasmid for predominant form of APP in the brains, were gifts from Dennis Selkoe & Tracy Young-Pearse(39). pCI-neo-ZIKV-prME is expression plasmid for PrM-E protein and was a gift from Dr. Asit Patnaik. pcDNA3 (Invitrogen) is a cloning vector used as controls for transfections.

Techniques: Transfection, Expressing, Plasmid Preparation, Control, Infection, Western Blot